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serum leptin  (R&D Systems)


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    R&D Systems serum leptin
    Serum Leptin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum leptin/product/R&D Systems
    Average 95 stars, based on 237 article reviews
    serum leptin - by Bioz Stars, 2026-05
    95/100 stars

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    Gene expression in adipose tissue of LRFI and HRFI bulls. (A) Volcano plot of adipose transcriptome between LRFI and HRFI bulls. (B) Bubble plot of enriched pathways and Sankey diagram of concerned genes. (C) Fold change of genes related to lipid metabolism in the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway (LRFI/HRFI). (D) Fold change of genes related to lipid metabolism in the cyclic adenosine monophosphate (cAMP) signaling pathway (LRFI/HRFI). LRFI = low residual feed intake; HRFI = high residual feed <t>intake;</t> <t>LEP</t> = <t>leptin</t> receptor; LEP = leptin; ACACA = acetyl-CoA carboxylase; PRKAA2 = protein kinase AMP-activated catalytic subunit α2; SREBP1 = sterol regulatory element-binding protein 1; CPT1B = carnitine palmitoyltransferase 1B; HSL = hormone-sensitive lipase; FASN = fatty acid synthase; SCD1 = stearoyl-CoA desaturase 1; CaMKKβ = calmodulin-dependent protein kinase kinase β; PRKAA1 = protein kinase AMP-activated catalytic subunit α1; CPT1A = carnitine palmitoyltransferase 1A; PPARα = peroxisome proliferator-activated receptor α; PRKACB = protein kinase cAMP-activated catalytic subunit β; PRKACA = protein kinase cAMP-activated catalytic subunit α; ACOX1 = acyl-CoA oxidase 1; ACOX3 = acyl-CoA oxidase 3; GNAS/GS = guanine nucleotide-binding protein G(s) subunit α; ADCY1 = adenylate cyclase 1; ADCYAP1R1 = adenylate cyclase activating polypeptide 1 receptor 1; ADCY10 = adenylate cyclase 10; ADCYAP1 = adenylate cyclase activating polypeptide 1.
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    Blockade of adipose sympathetic nerve does not prevent chronic stress-induced impairment of hepatic lipid homeostasis. (A-D) FFA, leptin, adiponectin, resistin levels in serum, n = 10 per group. (E) Experimental scheme. (F) Body weight, n= 5:6. (G) Food intake, n = 5:6. (H-K) Serum ALT, AST, TG and TC concentrations, n = 5:6. (L-O) FFA, leptin, adiponectin, resistin levels in serum, n = 5:6. (P) Liver weight and liver anatomy, n = 5:6. (Q) Liver TG, n = 5:6. (R) H&E staining (scale bars, 20 μm) and Oil Red O staining (scale bars, 20 μm) of liver sections, n = 5:6. (S) Relative mRNA levels in liver, n = 5:6. The data are presented as mean ± SEM. * P < 0.05. Unpaired two-tailed Student's t -test and one-way ANOVA were used for statistical analysis.

    Journal: International Journal of Biological Sciences

    Article Title: Chronic Stress Induces Hepatic Steatosis via Brain-Hepatic Sympathetic Axis Mediated Catecholamine Resistance

    doi: 10.7150/ijbs.126058

    Figure Lengend Snippet: Blockade of adipose sympathetic nerve does not prevent chronic stress-induced impairment of hepatic lipid homeostasis. (A-D) FFA, leptin, adiponectin, resistin levels in serum, n = 10 per group. (E) Experimental scheme. (F) Body weight, n= 5:6. (G) Food intake, n = 5:6. (H-K) Serum ALT, AST, TG and TC concentrations, n = 5:6. (L-O) FFA, leptin, adiponectin, resistin levels in serum, n = 5:6. (P) Liver weight and liver anatomy, n = 5:6. (Q) Liver TG, n = 5:6. (R) H&E staining (scale bars, 20 μm) and Oil Red O staining (scale bars, 20 μm) of liver sections, n = 5:6. (S) Relative mRNA levels in liver, n = 5:6. The data are presented as mean ± SEM. * P < 0.05. Unpaired two-tailed Student's t -test and one-way ANOVA were used for statistical analysis.

    Article Snippet: Serum leptin (E-EL-M3008), adiponectin (E-EL-M0002), resistin (E-EL-M3056), norepinephrine (NE, E-EL-0047), and hepatic cyclic adenosine monophosphate (cAMP, E-EL-0056) in liver extracts were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's intructions (Elabscience, Wuhan, China).

    Techniques: Staining, Two Tailed Test

    Gene expression in adipose tissue of LRFI and HRFI bulls. (A) Volcano plot of adipose transcriptome between LRFI and HRFI bulls. (B) Bubble plot of enriched pathways and Sankey diagram of concerned genes. (C) Fold change of genes related to lipid metabolism in the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway (LRFI/HRFI). (D) Fold change of genes related to lipid metabolism in the cyclic adenosine monophosphate (cAMP) signaling pathway (LRFI/HRFI). LRFI = low residual feed intake; HRFI = high residual feed intake; LEP = leptin receptor; LEP = leptin; ACACA = acetyl-CoA carboxylase; PRKAA2 = protein kinase AMP-activated catalytic subunit α2; SREBP1 = sterol regulatory element-binding protein 1; CPT1B = carnitine palmitoyltransferase 1B; HSL = hormone-sensitive lipase; FASN = fatty acid synthase; SCD1 = stearoyl-CoA desaturase 1; CaMKKβ = calmodulin-dependent protein kinase kinase β; PRKAA1 = protein kinase AMP-activated catalytic subunit α1; CPT1A = carnitine palmitoyltransferase 1A; PPARα = peroxisome proliferator-activated receptor α; PRKACB = protein kinase cAMP-activated catalytic subunit β; PRKACA = protein kinase cAMP-activated catalytic subunit α; ACOX1 = acyl-CoA oxidase 1; ACOX3 = acyl-CoA oxidase 3; GNAS/GS = guanine nucleotide-binding protein G(s) subunit α; ADCY1 = adenylate cyclase 1; ADCYAP1R1 = adenylate cyclase activating polypeptide 1 receptor 1; ADCY10 = adenylate cyclase 10; ADCYAP1 = adenylate cyclase activating polypeptide 1.

    Journal: Animal Nutrition

    Article Title: Differential lipid metabolism in beef cattle: A comparative study of high and low residual feed intake bulls

    doi: 10.1016/j.aninu.2025.03.019

    Figure Lengend Snippet: Gene expression in adipose tissue of LRFI and HRFI bulls. (A) Volcano plot of adipose transcriptome between LRFI and HRFI bulls. (B) Bubble plot of enriched pathways and Sankey diagram of concerned genes. (C) Fold change of genes related to lipid metabolism in the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway (LRFI/HRFI). (D) Fold change of genes related to lipid metabolism in the cyclic adenosine monophosphate (cAMP) signaling pathway (LRFI/HRFI). LRFI = low residual feed intake; HRFI = high residual feed intake; LEP = leptin receptor; LEP = leptin; ACACA = acetyl-CoA carboxylase; PRKAA2 = protein kinase AMP-activated catalytic subunit α2; SREBP1 = sterol regulatory element-binding protein 1; CPT1B = carnitine palmitoyltransferase 1B; HSL = hormone-sensitive lipase; FASN = fatty acid synthase; SCD1 = stearoyl-CoA desaturase 1; CaMKKβ = calmodulin-dependent protein kinase kinase β; PRKAA1 = protein kinase AMP-activated catalytic subunit α1; CPT1A = carnitine palmitoyltransferase 1A; PPARα = peroxisome proliferator-activated receptor α; PRKACB = protein kinase cAMP-activated catalytic subunit β; PRKACA = protein kinase cAMP-activated catalytic subunit α; ACOX1 = acyl-CoA oxidase 1; ACOX3 = acyl-CoA oxidase 3; GNAS/GS = guanine nucleotide-binding protein G(s) subunit α; ADCY1 = adenylate cyclase 1; ADCYAP1R1 = adenylate cyclase activating polypeptide 1 receptor 1; ADCY10 = adenylate cyclase 10; ADCYAP1 = adenylate cyclase activating polypeptide 1.

    Article Snippet: Serum leptin (LEP; ELISA kit CHE0053, Lot 20240122) levels were analyzed using a commercial kit (4A Biotech, Suzhou, Jiangsu, China) and a microplate reader (Multiskan MK3; Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Gene Expression, Binding Assay